Respiration – forms of anaerobic respiration

  • state where each of the phases of respiration (glycolysis, link reaction, Krebs cycle and oxidative phosphorylation) occurs in eukaryotic cells
  • explain that ATP is synthesised in substrate-linked reactions in glycolysis and in the Krebs cycle
  • explain that, when oxygen is available, pyruvate is converted into acetyl (2C) coenzyme A in the link reaction
  • describe investigations which could be performed to determine the effect of factors such as temperature and substrate concentration on the rate of respiration of yeast using a redox indicator (e.g. DCPIP or methylene blue)
  • distinguish between respiration in aerobic and anaerobic conditions in mammalian tissue and in yeast cells, contrasting the relative energy released by each (a detailed account of the total yield of ATP from the aerobic respiration of glucose is not required)
  • explain the production of a small yield of ATP from respiration in anaerobic conditions in yeast and in mammalian muscle tissue, including the concept of oxygen debt