Genetic engineering – locating & isolating genes

explain the use of genes for fluorescent or easily stained substances as markers in gene technology
explain the roles of restriction endonucleases, reverse transcriptase and ligases in genetic engineering
explain, in outline, how microarrays are used in the analysis of genomes and in detecting mRNA in studies of gene expression
outline the advantages of screening for genetic conditions (reference may be made to tests for specific genes such as those for breast cancer, BRCA1 and BRCA2, and genes for haemophilia, sickle cell anaemia, Huntington’s disease and cystic fibrosis)

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